Unlike the architecture and sculptural decoration of the Znamenskaya Church in Dubrovitsy, the program and artistic characteristics of its iconostasis have been largely overlooked. Of the entire ensemble, only ten icons have survived and been restored, while the others were recreated by contemporary artists based on photographs from the early 20th century. A rare icon of the Virgin Mary “Unfading Bloom,” has been discovered in the church collection, which, by many criteria, corresponds to the local image of the Virgin from the Znamenskaya Church, though it was significantly redone in the late 2010s. This article explores the unique features of this monument in the context of the development of the “Unfading Bloom” iconography in Russia. On the Dubrovitsy icon, the Virgin is depicted as the Queen of Heaven, holding a lily flower, with symbols that emphasize the apocalyptic theme. The article highlights the details of the depiction and technical innovations typical of the craftsmanship of the masters of the Armory Chamber from the late 17th century. In terms of drawing style and artistic techniques, the image aligns with works by K. I. Zolotarev, V. Poznansky, and especially I. P. Refusitsky, who, working on commissions from the royal family and metropolitan nobility, were influenced by Western European art.